In an attempt to investigate what really happened during Jehanne's trial, the Pope ordered a second trial in 1455. The new trial included testimony from over 100 witnesses who all knew Jehanne. These proceedings were well documented and preserved. According to the witness accounts, Jehanne had well developed breasts, but no pubic hair and that she never menstruated. These are strong indicators of a chromosomal abnormality known as testicular feminization. A person suffering from testicular feminization is chromosomally male, they have one X and one Y chromosome, but outwardly appears to be female. This gender confusion is due to a mutation in the X chromosome, which causes different hormones to be produced and results in the genitalia to appear to be female. The main symptoms are well developed breasts, little to no pubic hair, as well as the absence of menstruation (due the absence of internal female organs). All of these symptoms correspond to the testimonies that were given back in 1431.
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